RAD50 antibody

Catalog No. ABIN7606123

The Rabbit Monoclonal anti-RAD50 antibody has been validated for WB, ICC and IF. It is suitable to detect RAD50 in samples from Human, Mouse and Rat.

Quick Overview for RAD50 antibody (ABIN7606123)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: ACCG-18

Product Details anti-RAD50 Antibody

Purpose: Anti-Rad50 Monoclonal Antibody

Characteristics: Anti-Rad50 Monoclonal Antibody (ABIN7606123). Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Rad50 Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50

Background:

Synonyms: Ubiquitin-like modifier-activating enzyme ATG7,ATG12-activating enzyme E1 ATG7,Autophagy-related protein 7,APG7-like,hAGP7,Ubiquitin-activating enzyme E1-like protein,ATG7,APG7L,

Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Molecular Weight: 35 kDa

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus