RNF2 antibody

Catalog No. ABIN7606186

The Rabbit Monoclonal anti-RNF2 antibody has been validated for WB, IHC, IF, ICC and FACS. It is suitable to detect RNF2 in samples from Human, Mouse and Rat.

Quick Overview for RNF2 antibody (ABIN7606186)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: AEDC-18

Product Details anti-RNF2 Antibody

Purpose: Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody

Characteristics: Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody (ABIN7606186). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human RING2 / RING1B / RNF2 E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: RNF2

Background:

Synonyms: Vinculin,Metavinculin,MV,VCL,

Tissue Specificity: Metavinculin is muscle-specific.

Molecular Weight: 17 kDa

UniProt: Q99496