SKP1 antibody

Catalog No. ABIN7606300

The Rabbit Monoclonal anti-SKP1 antibody is suitable to detect SKP1 in samples from Human, Mouse and Rat. It has been validated for WB, IF, ICC and FACS.

Quick Overview for SKP1 antibody (ABIN7606300)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: ADDF-19

Product Details anti-SKP1 Antibody

Purpose: Anti-Skp1 Monoclonal Antibody

Characteristics: Anti-Skp1 Monoclonal Antibody (ABIN7606300). Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Skp1 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1

Background:

Synonyms: Microprocessor complex subunit DGCR8,DiGeorge syndrome critical region 8,DGCR8,C22orf12, DGCRK6,LP4941,

Tissue Specificity: Ubiquitously expressed. .

Molecular Weight: 501 kDa

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases