SMURF2 antibody

Catalog No. ABIN7606330

The Rabbit Monoclonal anti-SMURF2 antibody has been validated for WB. It is suitable to detect SMURF2 in samples from Human, Mouse and Rat.

Quick Overview for SMURF2 antibody (ABIN7606330)

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMURF2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: AECF-19

Product Details anti-SMURF2 Antibody

Purpose: Anti-SMURF 2 Rabbit Monoclonal Antibody

Characteristics: Anti-SMURF 2 Rabbit Monoclonal Antibody (ABIN7606330). Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SMURF 2 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then ly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Alternative Name: SMURF2

Background:

Synonyms: Neurogenic locus notch homolog protein 4,Notch 4,hNotch4,Notch 4 extracellular truncation,Notch 4 intracellular domain,NOTCH4,INT3,

Tissue Specificity: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

Molecular Weight: 41 kDa