Topoisomerase II alpha antibody

Catalog No. ABIN7606548

This Rabbit Monoclonal antibody specifically detects Topoisomerase II alpha in WB, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Topoisomerase II alpha antibody (ABIN7606548)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: FB-20

Product Details anti-Topoisomerase II alpha Antibody

Purpose: Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody

Characteristics: Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody (ABIN7606548). Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Topoisomerase II alpha

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Synonyms: DNA topoisomerase 2-alpha,5.99.1.3,DNA topoisomerase II, alpha isozyme,TOP2A,TOP2,

Tissue Specificity: Expressed in the excretory system, thyroid gland and Wilms tumors.

Molecular Weight: 28 kDa

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases