Recombinant Tyrosine Hydroxylase antibody (full length)

Catalog No. ABIN7607124

This Chicken Chimeric antibody specifically detects Tyrosine Hydroxylase in WB, IHC, ELISA, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Tyrosine Hydroxylase antibody (full length) (ABIN7607124)

Target: Tyrosine Hydroxylase (TH)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Chicken, Mouse

Clonality: Chimeric

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: LNC1

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: full length

Purpose: Anti-Tyrosine Hydroxylase (LNC1) Recombinant Chicken Chimeric mAb (LNC1)

Specificity: Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. No off-targets reported for the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.

Cross-Reactivity: Chicken, Frog, Human, Lizard, Microtus arvalis (Common vole) (Field vole), Mouse, Non-Human Primate, Rat, Zebrafish (Danio rerio)

Characteristics: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LNC1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LNC1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Purification: Purified by affinity chromatography.

Immunogen: Tyrosine Hydroxylase purified from PC12 cells

Isotype: IgY

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1X PBS, 0.05 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Molecular Weight: 59-63 kDa

Gene ID: 7054

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour