FGL2 antibody (AA 75-150)

Catalog No. ABIN727785

Cited in 1 publication. The Rabbit Polyclonal anti-FGL2 antibody (ABIN727785) specifically detects FGL2 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). The antibody is reactive with Mouse and Rat samples.

Quick Overview for FGL2 antibody (AA 75-150) (ABIN727785)

Target: FGL2 (Fibrinogen-Like 2 (FGL2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FGL2 Antibody

Binding Specificity: AA 75-150

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat FGL2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FGL2 antibody (ABIN727785)

Yang, Fujikado, Kolodin, Benoist, Mathis: "Immune tolerance. Regulatory T cells generated early in life play a distinct role in maintaining self-tolerance." in: Science (New York, N.Y.), Vol. 348, Issue 6234, pp. 589-94, (2015) (PubMed).

Target Details for FGL2

Target: FGL2 (Fibrinogen-Like 2 (FGL2))

Alternative Name: FGL2

Background:

Synonyms: Fibroleukin, Cytotoxic T-lymphocyte-specic protein, Fibrinogen-like protein 2, Fibrinogen like protein 2, Prothrombinase, Fibroleukin, pT49, T49, G3V7P2_RAT, Fibrinogen-like 2.

Background: FGL2 is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. It may play a role in physiologic functions at mucosal sites. It is constitutively expressed in cytotoxic T-cells. Lack of expression in other lymphoid- and nonlymphoid-derived cell lines suggested that expression of FGL2 may be restricted to lymphocytes. FGL2 is induced via a mechanism involving IFNG and components of the IFNG signaling pathway, including STAT1 and IRF1.

Gene ID: 84586

UniProt: G3V7P2