IL-8 antibody (AA 31-99)

Catalog No. ABIN728053

This Rabbit Polyclonal antibody specifically detects IL-8 in WB, ELISA, IHC (p), IHC (fro), IF (p) and IF (cc). It exhibits reactivity toward Human, Rabbit, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for IL-8 antibody (AA 31-99) (ABIN728053)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human, Rabbit, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-IL-8 Antibody

Binding Specificity: AA 31-99

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IL-8

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL-8 antibody (ABIN728053)

Xu, Huang, Zhang, Li, Zheng, Yu, Chu, Lin, Chen: "Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4167-74, (2017) (PubMed).

Zhang, Tseng, Zhang, Sirin, Corn, Li-Ning-Tapia, Troncoso, Davis, Pettaway, Ward, Frazier, Logothetis, Kolonin: "CXCL1 mediates obesity-associated adipose stromal cell trafficking and function in the tumour microenvironment." in: Nature communications, Vol. 7, pp. 11674, (2016) (PubMed).

Huang, Duan, Qian, Fan, Lv, Zhang, Han, Wu, Guo, Hu, Du, Chen, Jin: "IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma." in: Scientific reports, Vol. 6, pp. 36551, (2016) (PubMed).

Lu, Wei, Shao, Tang, Huang, Zhang, Yang, Jing: "Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles." in: Molecular biology reports, Vol. 40, Issue 4, pp. 3083-92, (2013) (PubMed).

Ma, Niu, Sun, Wang, Luo, Zhang, Wang: "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." in: Archives of toxicology, Vol. 86, Issue 6, pp. 849-56, (2012) (PubMed).

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8

Background:

Synonyms: IL8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-1C, T-cell chemotactic factor, CXCL8

Background: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-1-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Gene ID: 3576

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy