IL-6 antibody (AA 111-150)

Catalog No. ABIN728083

This Rabbit Polyclonal antibody specifically detects IL-6 in WB, ELISA, FACS, IF (p), IHC (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Cow and has been mentioned in 14+ publications.

Quick Overview for IL-6 antibody (AA 111-150) (ABIN728083)

Key Features:

• High quality IL-6 Primary Antibody for the detection of IL6.
• Reliable product with high standard validation data

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL-6 Antibody

Binding Specificity: AA 111-150

Cross-Reactivity: Cow, Human, Mouse, Rabbit, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse IL-6

Isotype: IgG

Product Specific Information:

What can the IL-6 Antibody ABIN728083 be used for?: This polyclonal IL-6 Antibody detects IL-6. The IL-6 Antibody has been validated for various applications and can be used for the detection of IL-6 and derivatives by Western Blotting, ELISA, Flow Cytometry, Immunofluorescence (Paraffin-embedded Sections), Immunohistochemistry (Paraffin-embedded Sections), Immunofluorescence (Cultured Cells), Immunohistochemistry (Frozen Sections).

What validation data is available for this IL-6 Antibody?: The primary antibody is referenced in 12 publications and is characterized by a proven, very high reliability. It has currently 6 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. IL-6 Antibody for the detection of IL-6 and derivatives.

What is the function of IL-6?: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL-6 antibody (ABIN728083)

Brockmann, Corkhill, Jaroslawska, Dege, Brockmann, Kociok, Joussen: "Systemic Rho-kinase inhibition using fasudil in mice with oxygen-induced retinopathy." in: Graefe's archive for clinical and experimental ophthalmology, Vol. 257, Issue 8, pp. 1699-1708, (2019) (PubMed).

Bai, Lv, Wang, Sun, Zhang: "LncRNA NEAT1 promotes inflammatory response and induces corneal neovascularization" in: Journal of molecular endocrinology, Vol. 61, Issue 4, pp. 231-239, (2019) (PubMed).

Sun, Li, Jia, Wang, Li, Hu, Tian, Yang, Xing, Zhang, Wang, Xu, Wang, Zhang, Ren: "Neuroprotection by IFN-γ via astrocyte-secreted IL-6 in acute neuroinflammation." in: Oncotarget, Vol. 8, Issue 25, pp. 40065-40078, (2018) (PubMed).

Haffner-Luntzer, Fischer, Prystaz, Liedert, Ignatius: "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." in: European journal of medical research, Vol. 22, Issue 1, pp. 23, (2017) (PubMed).

Prystaz, Kaiser, Kovtun, Haffner-Luntzer, Fischer, Rapp, Liedert, Strauss, Waetzig, Rose-John, Ignatius: "Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing." in: The American journal of pathology, Vol. 188, Issue 2, pp. 474-490, (2017) (PubMed).

Taskin, Gungor, Adali, Yay, Onder, Inceboz: "A Humanized Anti-Interleukin 6 Receptor Monoclonal Antibody, Tocilizumab, for the Treatment of Endometriosis in a Rat Model." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 23, Issue 5, pp. 662-9, (2016) (PubMed).

Kuracha, Thomas, Loggie, Govindarajan: "Patient-derived xenograft mouse models of pseudomyxoma peritonei recapitulate the human inflammatory tumor microenvironment." in: Cancer medicine, Vol. 5, Issue 4, pp. 711-9, (2016) (PubMed).

Zhu, Wang, Yang, Wang, Li, Zhou, Zhang: "Hematopoietic Effects of Paeoniflorin and Albiflorin on Radiotherapy-Induced Myelosuppression Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 5789381, (2016) (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, (2015) (PubMed).

Taskin, Topcu, Yay, Erken, Balcio?lu, Adali, Hismiogullar?: "Prevention of ovarian hyperstimulation syndrome in a rat model: comparison of the efficacy of tocilizumab with that of ranibizumab, cabergoline, and a gonadotropin-releasing hormone antagonist." in: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, pp. 1-6, (2015) (PubMed).

Luo, Wang, Li, Wei, Zhang, Liu, Wang, Niu, Wang: "Maternal bisphenol a diet induces anxiety-like behavior in female juvenile with neuroimmune activation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 140, Issue 2, pp. 364-73, (2015) (PubMed).

Takayanagi, Crawford, Kobayashi, Obama, Tsuji, Elliott, Hashimoto, Rizzo, Eguchi: "Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase." in: Clinical science (London, England : 1979), Vol. 126, Issue 11, pp. 785-94, (2014) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background:

Synonyms: Il-6, Interleukin-6, B-cell hybridoma growth factor, Interleukin HP-1, Il6

Background: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

Gene ID: 16193

UniProt: P08505

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome