Glutathione Reductase antibody (AA 421-522) (HRP)

Catalog No. ABIN728857

This Rabbit Polyclonal antibody specifically detects Glutathione Reductase in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for Glutathione Reductase antibody (AA 421-522) (HRP) (ABIN728857)

Target: Glutathione Reductase (GSR)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Reductase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Glutathione Reductase Antibody (HRP)

Binding Specificity: AA 421-522

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GSR

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: GLUR/glutathione reductase

Background:

Synonyms: glutathione reductase, GLUR, Glutathione reductase mitochondrial, GR, Gr1, GRase, GRD 1, GRD1, GSR, MGC78522.

Background: Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Gene ID: 2890

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis