Recombinant GM-CSF antibody

Catalog No. ABIN7630064

The Mouse Monoclonal anti-GM-CSF antibody has been validated for WB, IHC, IP, FACS, IF and ICC. It is suitable to detect GM-CSF in samples from Human.

Quick Overview for Recombinant GM-CSF antibody (ABIN7630064)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GM-CSF Antibody

Purpose: Recombinant Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)

Specificity: The antibody is a mouse monoclonal antibody raised against GM-CSF. It has been selected for its ability to recognize GM-CSF in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL, Immunohistochemistry: 5-20 μg/mL, Immunocytochemistry: 5-20 μg/mL, Flow cytometry:5 μg/mL, Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Colony Stimulating Factor 2, Granulocyte Macrophage

Background: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-Macrophage Colony Stimulating Factor

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin