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Recombinant His Tag antibody

This Mouse Monoclonal antibody specifically detects His Tag in WB, IP, IHC, IF, FACS and ICC. It exhibits reactivity toward .
Catalog No. ABIN7630081

Quick Overview for Recombinant His Tag antibody (ABIN7630081)

Target

See all His Tag Antibodies
His Tag

Antibody Type

Recombinant Antibody

Reactivity

Please inquire

Host

  • 106
  • 65
  • 11
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  • 1
Mouse

Clonality

  • 121
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Monoclonal

Conjugate

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This His Tag antibody is un-conjugated

Application

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Purpose

    Recombinant Antibody to Hexa Histidine (H6)

    Specificity

    The antibody is a mouse monoclonal antibody raised against H6. It has been selected for its ability to recognize H6 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    His Tag

    Alternative Name

    Hexa Histidine

    Target Type

    Tag

    Background

    6xHis, His-Tag
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