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Serotonin antibody (FITC)

The Rabbit Polyclonal anti-Serotonin antibody has been validated for IHC, WB, ICC and IF. It is suitable to detect Serotonin in samples from Various Species.
Catalog No. ABIN7630181

Quick Overview for Serotonin antibody (FITC) (ABIN7630181)

Target

See all Serotonin (5-HT) Antibodies
Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivity

Various Species

Host

  • 23
  • 4
  • 1
  • 1
Rabbit

Clonality

  • 25
  • 3
Polyclonal

Conjugate

  • 11
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Serotonin antibody is conjugated to FITC

Application

  • 13
  • 13
  • 9
  • 8
  • 7
  • 6
  • 6
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • Purpose

    FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against 5-HT. It has been selected for its ability to recognize 5-HT in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Various Species

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    PAA808Ge01Polyclonal Antibody to 5Hydroxytryptamine (5HT)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

    Alternative Name

    5-Hydroxytryptamine

    Target Type

    Chemical

    Background

    5HT, Serotonin, N-Methyl-Gamma
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