C5A antibody (Biotin)

Catalog No. ABIN7631059

This Mouse Monoclonal antibody specifically detects C5A in WB, IHC and ICC. It exhibits reactivity toward Mouse.

Quick Overview for C5A antibody (Biotin) (ABIN7631059)

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5A antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-C5A Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a)

Specificity: The antibody is a mouse monoclonal antibody raised against C5a. It has been selected for its ability to recognize C5a in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA388Mu21Monoclonal Antibody to Complement Component 5a (C5a)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for C5A

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement Component 5a

UniProt: P06684

Pathways: Complement System, Carbohydrate Homeostasis