C4B antibody (Biotin)

Catalog No. ABIN7631060

The Rabbit Polyclonal anti-C4B antibody has been validated for WB, IHC and ICC. It is suitable to detect C4B in samples from Mouse.

Quick Overview for C4B antibody (Biotin) (ABIN7631060)

Target: C4B (C4b) (Complement Component C4b (C4b))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C4B antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-C4B Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Complement C4-B (C4B)

Specificity: The antibody is a rabbit polyclonal antibody raised against C4B. It has been selected for its ability to recognize C4B in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB305Mu01Polyclonal Antibody to Complement C4B (C4B)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for C4B

Target: C4B (C4b) (Complement Component C4b (C4b))

Alternative Name: Complement C4-B

Background: CO4, CPAMD3, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, Complement component 4B, Chido blood group

UniProt: P01029

Pathways: Complement System