CD35 antibody (Biotin)

Catalog No. ABIN7631065

This Rabbit Polyclonal antibody specifically detects CD35 in IHC, WB and ICC. It exhibits reactivity toward Human.

Quick Overview for CD35 antibody (Biotin) (ABIN7631065)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD35 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-CD35 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Complement Receptor 1, Erythrocyte (CR1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CR1. It has been selected for its ability to recognize CR1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB123Hu01Polyclonal Antibody to Complement Receptor 1, Erythrocyte (CR1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: Complement Receptor 1, Erythrocyte

Background: CD35, C3BR, KN, C3b/C4b Receptor,Including Knops Blood Group System, Immune Adherence Receptor

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity