EGF antibody (Biotin)

Catalog No. ABIN7631284

This Rabbit Polyclonal antibody specifically detects EGF in WB, IHC and ICC. It exhibits reactivity toward Pig.

Quick Overview for EGF antibody (Biotin) (ABIN7631284)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-EGF Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Epidermal Growth Factor (EGF)

Specificity: The antibody is a rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA560Po01Polyclonal Antibody to Epidermal Growth Factor (EGF)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor

Background: URG, Beta-Urogastrone, Pro-epidermal growth factor

UniProt: Q00968

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation