EGF antibody (Biotin)

Catalog No. ABIN7702924

The Biotin-conjugated Mouse Monoclonal anti-EGF antibody (Clone C10) (ABIN7702924) specifically detects EGF in WB, IHC and ICC. The antibody is reactive with Rat samples.

Quick Overview for EGF antibody (Biotin) (ABIN7702924)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGF antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: C10

Product Details anti-EGF Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Epidermal Growth Factor (EGF)

Specificity: The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA560Ra21Monoclonal Antibody to Epidermal Growth Factor (EGF)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor

Background: URG, Beta-Urogastrone, Pro-epidermal growth factor

UniProt: P07522

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation