Fibronectin antibody (Biotin)
Quick Overview for Fibronectin antibody (Biotin) (ABIN7631430)
Target
See all Fibronectin AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- Biotin-Linked Polyclonal Antibody to Fibronectin (FN)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Human, Mouse
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- PAA037Ra01Polyclonal Antibody to Fibronectin (FN)
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Isotype
- IgG
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Application Notes
- Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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- Fibronectin
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Background
- FN1, CIG, FINC, LETS, MSF, GFND2, Anastellin, Migration-Stimulating Factor, Cold-Insoluble Globulin, Large, External, Transformation-Sensitive Protein
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UniProt
- P04937
Target
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