ITK antibody (Biotin)

Catalog No. ABIN7631885

The Rabbit Polyclonal anti-ITK antibody has been validated for WB, IHC and ICC. It is suitable to detect ITK in samples from Mouse.

Quick Overview for ITK antibody (Biotin) (ABIN7631885)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITK antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ITK Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to IL2 Inducible T-Cell Kinase (ITK)

Specificity: The antibody is a rabbit polyclonal antibody raised against ITK. It has been selected for its ability to recognize ITK in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAH774Mu01Polyclonal Antibody to IL2 Inducible TCell Kinase (ITK)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: IL2 Inducible T-Cell Kinase

Background: PSCTK2, EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk

UniProt: Q03526

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway