IL12A antibody (Biotin)

Catalog No. ABIN7632089

The Rabbit Polyclonal anti-IL12A antibody has been validated for WB, IHC and ICC. It is suitable to detect IL12A in samples from Mouse.

Quick Overview for IL12A antibody (Biotin) (ABIN7632089)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12A antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-IL12A Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Interleukin 12A (IL12A)

Specificity: The antibody is a rabbit polyclonal antibody raised against IL12A. It has been selected for its ability to recognize IL12A in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA059Mu01Polyclonal Antibody to Interleukin 12A (IL12A)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin 12A

Background: P35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1

UniProt: P43431

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation