MAFA antibody

Catalog No. ABIN729898

This Rabbit Polyclonal antibody specifically detects MAFA in WB, IF (p) and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAFA antibody (ABIN729898)

Target: MAFA (Transcription Factor MafA (MAFA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAFA Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Mafa

Isotype: IgG

Application Details

Application Notes: WB: (1:100-1000), IHC-P: (1:100-500), IF(IHC-P): (1:50-200)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MAFA

Target: MAFA (Transcription Factor MafA (MAFA))

Alternative Name: Mafa

Background:

Synonyms: Mafa homolog, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A,Pancreatic beta-cell-specic transcriptional activator,avianV-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA_MOUSE.

Background: Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Gene ID: 389692

Pathways: Hormone Transport