MUC16 antibody (Biotin)

Catalog No. ABIN7632572

The Rabbit Polyclonal anti-MUC16 antibody has been validated for WB, IHC and ICC. It is suitable to detect MUC16 in samples from Mouse.

Quick Overview for MUC16 antibody (Biotin) (ABIN7632572)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC16 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-MUC16 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Carbohydrate Antigen 125 (CA125)

Specificity: The antibody is a rabbit polyclonal antibody raised against CA125. It has been selected for its ability to recognize CA125 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA154Mu01Polyclonal Antibody to Carbohydrate Antigen 125 (CA125)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: Carbohydrate Antigen 125

Background: MUC16, Cancer Antigen 125, Mucin 16,Cell Surface Associated, Ovarian Cancer-Related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125

NCBI Accession: XM_911929