PKC epsilon antibody (Biotin)

Catalog No. ABIN7633001

The Rabbit Polyclonal anti-PKC epsilon antibody has been validated for WB, IHC and ICC. It is suitable to detect PKC epsilon in samples from Human.

Quick Overview for PKC epsilon antibody (Biotin) (ABIN7633001)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC epsilon antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PKC epsilon Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Protein Kinase C Epsilon (PKCe)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKCe. It has been selected for its ability to recognize PKCe in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: Protein Kinase C Epsilon

Background: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling