SMAD2 antibody (Biotin)

Catalog No. ABIN7633333

This Rabbit Polyclonal antibody specifically detects SMAD2 in WB, IHC and ICC. It exhibits reactivity toward Mouse.

Quick Overview for SMAD2 antibody (Biotin) (ABIN7633333)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SMAD2 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)

Specificity: The antibody is a rabbit polyclonal antibody raised against Smad2. It has been selected for its ability to recognize Smad2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Mothers Against Decapentaplegic Homolog 2

Background: JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2

UniProt: Q62432

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus