TLR4 antibody (Biotin)

Catalog No. ABIN7633522

This Rabbit Polyclonal antibody specifically detects TLR4 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for TLR4 antibody (Biotin) (ABIN7633522)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-TLR4 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

Specificity: The antibody is a rabbit polyclonal antibody raised against TLR4. It has been selected for its ability to recognize TLR4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: Toll Like Receptor 4

Background: CD284, TOLL, HToll

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins