UCP1 antibody (Biotin)

Catalog No. ABIN7633690

This Rabbit Polyclonal antibody specifically detects UCP1 in WB, IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for UCP1 antibody (Biotin) (ABIN7633690)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-UCP1 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: Uncoupling Protein 1, Mitochondrial

Background: SLC25A7, UCP, Thermogenin, Solute carrier family 25 member 7

UniProt: P04633

Pathways: Proton Transport, Brown Fat Cell Differentiation