APEX1 antibody

Catalog No. ABIN7634962

This Rabbit Polyclonal antibody specifically detects APEX1 in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for APEX1 antibody (ABIN7634962)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-APEX1 Antibody

Purpose: Polyclonal Antibody to Apurinic/Apyrimidinic Endonuclease 1 (APEX1)

Specificity: The antibody is a rabbit polyclonal antibody raised against APEX1. It has been selected for its ability to recognize APEX1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1

Background: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase

UniProt: P28352

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus