ATG5 antibody

Catalog No. ABIN7635116

The Rabbit Polyclonal anti-ATG5 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ATG5 in samples from Human.

Quick Overview for ATG5 antibody (ABIN7635116)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ATG5 Antibody

Purpose: Polyclonal Antibody to Autophagy Related Protein 5 (ATG5)

Specificity: The antibody is a rabbit polyclonal antibody raised against ATG5. It has been selected for its ability to recognize ATG5 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPL221Hu01Recombinant Autophagy Related Protein 5 (ATG5)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: ASP, APG5, APG5L, hAPG5, Apoptosis-specific protein, Autophagy protein 5

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy