DFFA antibody

Catalog No. ABIN7638046

This Rabbit Polyclonal antibody specifically detects DFFA in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for DFFA antibody (ABIN7638046)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DFFA Antibody

Purpose: Polyclonal Antibody to DNA Fragmentation Factor Subunit Alpha (DFFa)

Specificity: The antibody is a rabbit polyclonal antibody raised against DFFa. It has been selected for its ability to recognize DFFa in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFa

Background: DFF-45, DFF1, ICAD, DNA Fragmentation Factor,45 kDa,Alpha Polypeptide, Inhibitor of CAD

UniProt: O54786

Pathways: Apoptosis, Caspase Cascade in Apoptosis