Endothelin 1 antibody (APC)

Catalog No. ABIN7638288

This anti-Endothelin 1 antibody is a Guinea Pig Polyclonal antibody detecting Endothelin 1 in WB, IHC, IP and ICC. Suitable for Rabbit.

Quick Overview for Endothelin 1 antibody (APC) (ABIN7638288)

Target: Endothelin 1 (EDN1)

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Endothelin 1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Endothelin 1 Antibody (APC)

Purpose: Polyclonal Antibody to Endothelin 1 (EDN1)

Specificity: The antibody is a cavia polyclonal antibody raised against EDN1. It has been selected for its ability to recognize EDN1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: EDN1

Background: ET1, PPET1, Preproendothelin-1, Big endothelin-1

UniProt: P29560

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size