Glypican 3 antibody

Catalog No. ABIN7639714

This Mouse Monoclonal antibody specifically detects Glypican 3 in WB, IHC, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for Glypican 3 antibody (ABIN7639714)

Target: Glypican 3 (GPC3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C12

Product Details anti-Glypican 3 Antibody

Purpose: Monoclonal Antibody to Glypican 3 (GPC3)

Specificity: The antibody is a mouse monoclonal antibody raised against GPC3. It has been selected for its ability to recognize GPC3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPA971Hu01Recombinant Glypican 3 (GPC3)

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: DGSX, OCI5, SDYS, SGB, SGBS1, MXR7, Glypican Proteoglycan 3, GTR2-2, Intestinal protein OCI-5, Secreted glypican-3

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process