GRP78 antibody

Catalog No. ABIN7640027

The Mouse Monoclonal anti-GRP78 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect GRP78 in samples from Mouse.

Quick Overview for GRP78 antibody (ABIN7640027)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C2

Product Details anti-GRP78 Antibody

Purpose: Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

Specificity: The antibody is a mouse monoclonal antibody raised against HSPA5. It has been selected for its ability to recognize HSPA5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPC343Mu01Recombinant Heat Shock 70kDa Protein 5 (HSPA5)

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5

Background: MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78

UniProt: P20029

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling