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Cytokeratin 18 antibody

This anti-Cytokeratin 18 antibody is a Rabbit Polyclonal antibody detecting Cytokeratin 18 in WB, IHC, IP and ICC. Suitable for Pig.
Catalog No. ABIN7641708

Quick Overview for Cytokeratin 18 antibody (ABIN7641708)

Target

See all Cytokeratin 18 (KRT18) Antibodies
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity

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Pig

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Cytokeratin 18 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Purpose

    Polyclonal Antibody to Cytokeratin 18 (CK18)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Human, Pig

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    RPB231Po01Recombinant Cytokeratin 18 (CK18)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    Preservative

    Dithiothreitol (DTT), ProClin

    Precaution of Use

    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    Alternative Name

    CK18

    Background

    KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

    UniProt

    A8D349

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis
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