Cytokeratin 18 antibody

Catalog No. ABIN7641710

The Mouse Monoclonal anti-Cytokeratin 18 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Cytokeratin 18 in samples from Human.

Quick Overview for Cytokeratin 18 antibody (ABIN7641710)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: E6

Product Details anti-Cytokeratin 18 Antibody

Purpose: Monoclonal Antibody to Cytokeratin 18 (CK18)

Specificity: The antibody is a mouse monoclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPB231Hu01Recombinant Cytokeratin 18 (CK18)

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry: 10 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: CK18

Background: KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis