PDE5A antibody

Catalog No. ABIN7644270

This anti-PDE5A antibody is a Rabbit Polyclonal antibody detecting PDE5A in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for PDE5A antibody (ABIN7644270)

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDE5A Antibody

Purpose: Polyclonal Antibody to Phosphodiesterase 5A, cGMP Specific (PDE5A)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDE5A. It has been selected for its ability to recognize PDE5A in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PDE5A

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: PDE5A

Background: CGB-PDE, cGMP-binding cGMP-specific phosphodiesterase, cGMP-specific 3',5'-cyclic phosphodiesterase

UniProt: O76074

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling