PFKL antibody

Catalog No. ABIN7644277

This Rabbit Polyclonal antibody specifically detects PFKL in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for PFKL antibody (ABIN7644277)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PFKL Antibody

Purpose: Polyclonal Antibody to Phosphofructokinase, Liver (PFKL)

Specificity: The antibody is a rabbit polyclonal antibody raised against PFKL. It has been selected for its ability to recognize PFKL in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPD406Ra01Recombinant Phosphofructokinase, Liver (PFKL)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.38 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background: PFK-B, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase

UniProt: P30835

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect