SQSTM1 antibody

Catalog No. ABIN7645948

This Mouse Monoclonal antibody specifically detects SQSTM1 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for SQSTM1 antibody (ABIN7645948)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D4

Product Details anti-SQSTM1 Antibody

Purpose: Monoclonal Antibody to Sequestosome 1 (SQSTM1)

Specificity: The antibody is a mouse monoclonal antibody raised against SQSTM1. It has been selected for its ability to recognize SQSTM1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPD198Hu01Recombinant Sequestosome 1 (SQSTM1)

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: OSIL, PDB3, ZIP3, p62, p62B, p60, A170, Paget Disease Of Bone 3, Oxidative Stress Induced Like, EBI3-associated protein of 60 kDa, Ubiquitin-binding protein p62

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy