KAP1 antibody

Catalog No. ABIN7647542

The Rabbit Polyclonal anti-KAP1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect KAP1 in samples from Mouse.

Quick Overview for KAP1 antibody (ABIN7647542)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-KAP1 Antibody

Purpose: Polyclonal Antibody to Transcriptional Intermediary Factor 1 Beta (TIF1b)

Specificity: The antibody is a rabbit polyclonal antibody raised against TIF1b. It has been selected for its ability to recognize TIF1b in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPF057Mu01Recombinant Transcriptional Intermediary Factor 1 Beta (TIF1b)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.34 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TIF1b

Background: TRIM28, KAP1, RNF96, TF1B, Tripartite Motif Containing 28, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, RING finger protein 96, Nuclear corepressor KAP-1

UniProt: Q62318

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus