Vinculin antibody

Catalog No. ABIN7648106

The Mouse Monoclonal anti-Vinculin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Vinculin in samples from Human.

Quick Overview for Vinculin antibody (ABIN7648106)

Target: Vinculin (VCL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-Vinculin Antibody

Purpose: Monoclonal Antibody to Vinculin (VCL)

Specificity: The antibody is a mouse monoclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPB839Hu01Recombinant Vinculin (VCL)

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: MVCL, Metavinculin

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2