OGG1 antibody (CF®740)

Catalog No. ABIN7649057

The Mouse Monoclonal anti-OGG1 antibody has been validated for IHC and IHC (fp). It is suitable to detect OGG1 in samples from Human.

Quick Overview for OGG1 antibody (CF®740) (ABIN7649057)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OGG1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CPTC-OGG1-1

Product Details anti-OGG1 Antibody (CF®740)

Purpose: 8-Oxoguanine DNA Glycosylase(CPTC-OGG1-1), CF740 conjugate

Characteristics: 8-Oxoguanine DNA Glycosylase (OGG1), otherwise known as N-Glycosylase/DNA lyase, is the enzyme responsible for the excision of 8-oxoguanine (8-oxoG), a mutagenic base by-product which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversion mutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Defects in OGG1 may be a cause of renal cell carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human OGG1 protein

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa or Jurkat cells. Kidney, skin or lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: N-Glycosylase/DNA lyase

Background:

Synonyms: 8-oxoguanine DNA glycosylase, HMMH, HOGG1, MUTM, OGH1

Gene Symbol: OGG1

Molecular Weight: 39 kDa

Gene ID: 4968

UniProt: O15527

Pathways: DNA Damage Repair