AMPD3 antibody (CF®740)

Catalog No. ABIN7649180

The Mouse Monoclonal anti-AMPD3 antibody has been validated for IHC and IHC (fp). It is suitable to detect AMPD3 in samples from Human.

Quick Overview for AMPD3 antibody (CF®740) (ABIN7649180)

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMPD3 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: AMPD3-901

Product Details anti-AMPD3 Antibody (CF®740)

Purpose: Adenosine Monophosphate Deaminase 3 (AMPD3)(AMPD3/901), CF740 conjugate

Characteristics: Adenosine Monophosphate Deaminase, isoform E (AMPD3) is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. It has 767 amino acids, a MW of ~ 90 kDa, and is assigned an EC 3.5.4.6. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human AMDP3 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunocytochemistry 1-2.0 μg/mL. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 1-2 μg/million cells in 0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: RBC. Fetal liver, Spleen or Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for AMPD3

Target: AMPD3 (Adenosine Monophosphate Deaminase 3 (AMPD3))

Alternative Name: Adenosine Monophosphate Deaminase-3

Background:

Synonyms: AMPD3, Erythrocyte AMP Deaminase, AMP Deaminase 3

Gene Symbol: AMPD3

Tissue Expression: Red blood cells

Molecular Weight: 90 kDa

Gene ID: 272

UniProt: Q01432