ACTH antibody (AA 25-39) (CF®740)

Catalog No. ABIN7649235

This anti-ACTH antibody is a Mouse Monoclonal antibody detecting ACTH in IHC and IHC (fp). Suitable for Human.

Quick Overview for ACTH antibody (AA 25-39) (CF®740) (ABIN7649235)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CLIP-1449

Product Details anti-ACTH Antibody (CF®740)

Binding Specificity: AA 25-39

Purpose: ACTH (Adrenocorticotrophic Hormone) (C-Terminus) (CLIP/1449), CF740 conjugate

Characteristics: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide corresponding to aa25-39 of human ACTH (NGAEDESAEAFPLEF)

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH

Background:

Synonyms: Pro-opiomelanocortin (POMC), Adrenocorticotropin, Adrenocorticotropic hormone, Melanocyte Stimulating Hormone (MSH), Melanotropin, beta-Endorphin, Lipotropin, Met-Enkephalin, POC

Gene Symbol: POMC

Molecular Weight: ACTH is ~5 kDa

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity