Recombinant ACTH antibody (AA 25-39)

Catalog No. ABIN7649258

The Rabbit Monoclonal anti-ACTH antibody has been validated for . It is suitable to detect ACTH in samples from Human.

Quick Overview for Recombinant ACTH antibody (AA 25-39) (ABIN7649258)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Please inquire

Clone: CLIP-2859R

Product Details anti-ACTH Antibody

Binding Specificity: AA 25-39

Purpose: ACTH (Adrenocorticotrophic Hormone) (C-Terminus)(CLIP/2859R)

Characteristics: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH), does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide corresponding to aa25-39 of human ACTH (NGAEDESAEAFPLEF)

Isotype: IgG

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH

Background:

Synonyms: Pro-opiomelanocortin (POMC), Adrenocorticotropin, Adrenocorticotropic hormone, Melanocyte Stimulating Hormone (MSH), Melanotropin, beta-Endorphin, Lipotropin, Met-Enkephalin, POC

Gene Symbol: POMC

Molecular Weight: ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity