Annexin a1 antibody (CF®568)

Catalog No. ABIN7649527

This Mouse Monoclonal antibody specifically detects Annexin a1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Annexin a1 antibody (CF®568) (ABIN7649527)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin a1 antibody is conjugated to CF®568

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ANXA1-1672

Product Details anti-Annexin a1 Antibody (CF®568)

Purpose: Annexin A1 / (Hairy Cell Leukemia Marker)(ANXA1/1672), CF568 conjugate

Characteristics: Annexin A1 belongs to the annexin family. It plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. In resting conditions Annexin A1 is localized to the cytoplasm. Upon activation it is mobilized to the membrane and secreted. Annexin A1 and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. Expression of the ANXA1 gene is upregulated in hairy cell leukemia (HCL). Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Annexin A1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 1-2 μg/million cells, ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HeLa. A431 and K562 cells, human esophagus, placenta and prostate tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: Annexin A1

Background:

Synonyms: Annexin-1, Calpactin-2, Chromobindin-9, Phospholipase A2 inhibitory protein, LPC1, p35

Gene Symbol: ANXA1

Molecular Weight: 35 kDa

Gene ID: 301

UniProt: P04083

Pathways: Hormone Transport