BCL6 antibody (AA 256-389) (Biotin)

Catalog No. ABIN7649959

This Mouse Monoclonal antibody specifically detects BCL6 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for BCL6 antibody (AA 256-389) (Biotin) (ABIN7649959)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL6 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BCL6-1718

Product Details anti-BCL6 Antibody (Biotin)

Binding Specificity: AA 256-389

Purpose: Bcl-6 (Follicular Lymphoma Marker) (BCL6/1718), Biotin conjugate

Characteristics: Bcl-6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. It forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Bcl-6 allows GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. Antibodies to Bcl-6 are helpful in a number of diagnostic settings, for example in the differential diagnosis of small B-cell lymphoma, and in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human bcl-6 protein fragment (aa256-389)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Raji or Ramos cells. Tonsil or Hodgkin lymphoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Background:

Synonyms: Bcl-6, B-Cell Lymphoma 6 Protein, B-cell lymphoma 5 protein, Zinc Finger Protein 51, zinc finger transcription factor BCL6S, BCL6 Transcription Repressor

Gene Symbol: BCL6

Molecular Weight: 95 kDa

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus