Recombinant Biotin antibody (CF®740)

Catalog No. ABIN7650069

The CF®740-conjugated Rabbit Monoclonal anti-Biotin antibody (Clone BTN-2032R) (ABIN7650069) specifically detects Biotin in IHC and IHC (fp). The antibody is reactive with Various Species samples.

Quick Overview for Recombinant Biotin antibody (CF®740) (ABIN7650069)

Target: Biotin

Antibody Type: Recombinant Antibody

Reactivity: Various Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Biotin antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BTN-2032R

Product Details anti-Biotin Antibody (CF®740)

Purpose: Biotin (Vitamin B7 or Vitamin H) (BTN/2032R), CF740 conjugate

Characteristics: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Biotinylated sheep immunoglobulin

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. In situ Nucleic Acid Hybridization. No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.. Western blotting. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Biotinylated proteins in solution or on tissues

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background:

Synonyms: Vitamin B7 or Vitamin H

Gene Symbol: Not Applicable

Molecular Weight: 244Da