BMI1 antibody (AA 142-326) (CF®647)

Catalog No. ABIN7650085

This Mouse Monoclonal antibody specifically detects BMI1 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for BMI1 antibody (AA 142-326) (CF®647) (ABIN7650085)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BMI1-2690

Product Details anti-BMI1 Antibody (CF®647)

Binding Specificity: AA 142-326

Purpose: BMI1 (Oncogene and Stem Cell Marker)(BMI1/2690), CF647 conjugate

Characteristics: The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 142-326) of human BMI1 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa or Jurkat cells. Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background:

Synonyms: BMI1, Polycomb complex protein BMI1, B lymphoma Mo MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, Polycomb group ring finger 4, RING finger protein 51

Gene Symbol: BMI1

Molecular Weight: ~41 kDa

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy