BMI1 antibody (AA 142-326)

Catalog No. ABIN7650107

The Mouse Monoclonal anti-BMI1 antibody has been validated for WB, IHC, IF and IHC (fp). It is suitable to detect BMI1 in samples from Human and Mouse.

Quick Overview for BMI1 antibody (AA 142-326) (ABIN7650107)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BMI1-2823

Product Details anti-BMI1 Antibody

Binding Specificity: AA 142-326

Purpose: BMI1 (Oncogene and Stem Cell Marker)(BMI1/2823)

Characteristics: The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 142-326) of human BMI1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa or Jurkat cells. Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Preservative: Without preservative

Storage: -20 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background:

Synonyms: BMI1, Polycomb complex protein BMI1, B lymphoma Mo MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, Polycomb group ring finger 4, RING finger protein 51

Gene Symbol: BMI1

Molecular Weight: ~41 kDa

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy