CLEC9A antibody (CF®647)

Catalog No. ABIN7650192

The CF®647-conjugated Rat Monoclonal anti-CLEC9A antibody (Clone 1F6) (ABIN7650192) specifically detects CLEC9A in FACS and Func. The antibody is reactive with Mouse samples.

Quick Overview for CLEC9A antibody (CF®647) (ABIN7650192)

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CLEC9A antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: 1F6

Product Details anti-CLEC9A Antibody (CF®647)

Purpose: CLEC9A(1F6), CF647 conjugate

Characteristics: This Mab recognizes Mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a and the CD103 DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31( ) dendritic cells and on a small subset of CD14( ) and CD16(-) monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: 0.5-1 μg/million cells, Western blot 0.5-1 μg/mL, Immunofluorescence: 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Mouse spleen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CLEC9A

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Alternative Name: CLEC9A

Background: C type lectin domain family 9, member A, C-type lectin domain family 8 member A, CLEC9A, DNGR1, HEEE9341, PRO34046, UNQ9341, CD370

Molecular Weight: 27 kDa

Gene ID: 232414